The main difference between 24/6 and 26/6 staples lies in the wire gauge; 24/6 staples have a thicker wire (lower gauge number) than 26/6 staples, while both have the same shank length of 6mm. The first number in the designation (24 or 26) indicates the wire gauge, with smaller numbers representing thicker wire, according to Eastpoint Global Ltd and AOS Online.